[1]程建平,韦水翠,何则强.双水相纯化湖南黑茶茶多酚工艺优化[J].湖南城市学院学报(自然科学版),2019,28(5):65-69.[doi:10.3969/j.issn.1672-7304.2019.05.0014]
 CHENG Jianpingl,WEI Shuicui,HE Zeqiang*.Optimization of Purification Technology of Tea Polyphenols from Hunan Dark Tea by Aqueous Two-phase System[J].,2019,28(5):65-69.[doi:10.3969/j.issn.1672-7304.2019.05.0014]
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双水相纯化湖南黑茶茶多酚工艺优化()
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《湖南城市学院学报(自然科学版)》[ISSN:1006-6977/CN:61-1281/TN]

卷:
28
期数:
2019年5期
页码:
65-69
栏目:
化学与材料
出版日期:
2019-09-20

文章信息/Info

Title:
Optimization of Purification Technology of Tea Polyphenols from Hunan Dark Tea by Aqueous Two-phase System
文章编号:
1672–7304(2019)05–0065–05
作者:
程建平韦水翠何则强
1. 益阳市赫山区教育局,湖南 益阳 413000;2. 吉首大学 生物资源与环境科学学院,湖南 吉首 416000;3. 湖南城市学院 黑茶金花湖南省重点实验室,湖南 益阳 413000
Author(s):
CHENG Jianpingl WEI Shuicui2 HE Zeqiang3*
1. Heshan District Education Bureau, Yiyang, Hunan 413000, China; 2. College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan 416000, China; 3. Hunan Provincial Key Lab of Dark Tea and Jin-hua, Hunan City University, Yiyang, Hunan 413000, China
关键词:
双水相体系湖南黑茶茶多酚分配系数萃取率
Keywords:
aqueous two-phase system Hunan Dark Tea tea polyphenols the partition coefficient extraction rate
分类号:
H282.4;O629.9
DOI:
10.3969/j.issn.1672-7304.2019.05.0014
文献标志码:
A
摘要:
选用PEG-(NH4)2SO4双水相体系对湖南黑茶茶多酚进行分离纯化﹒通过单因素实验考察PEG的平均分子量与质量分数、硫酸铵质量分数、提取液体积、体系pH值和温度等多种因素对茶多酚纯化的分配系数以及萃取率的影响,并选择正交实验对茶多酚纯化工艺条件进行进一步优化﹒实验表明,双水相纯化湖南黑茶茶多酚的最佳工艺条件为:10.0 g双水相体系中,选用平均分子量为600、质量分数为30%的PEG,质量分数为21%的(NH4)2SO4,体系的提取液体积为2.7 mL,pH=5.5,温度为25 ℃﹒在此最优工艺条件下,可得双水相纯化湖南黑茶茶多酚的分配系数为34.50,萃取率可达97.57%﹒
Abstract:
The tea polyphenols from Hunan Dark Tea were separated and purified by PEG-(NH4)2SO4 aqueous two-phase system. The effects of the average molecular weight and mass fraction of PEG, the mass fraction of ammonium sulfate, the volume of extraction liquid, the pH and temperature of the system on the partition coefficient and extraction rate of tea polyphenols were investigated by single factor experiment. Orthogonal experiments were selected to further optimize the purification conditions of the tea polyphenols. The experiments showed that the optimum technological conditions for the purification of the tea polyphenols from Hunan Dark Tea by aqueous two-phase method were as follows: in 10.0 g aqueous two-phase system, PEG with an average molecular weight of 600 and a mass fraction of 30% was selected, and the mass fraction was 21% (NH4)2SO4, the volume of extraction liquid was 2.7 mL, the pH was 5.5, and the temperature was 25 ℃. Under the optimal purification conditions, the partition coefficient of tea polyphenols purified by aqueous two-phase method was 34.50, and the extraction rate was 97.57%.

备注/Memo

备注/Memo:
收稿日期:2019-06-10 基金项目:国家自然科学基金资助项目(51672104,51862008);湖南省自然科学基金资助项目(2017JJ2216);湘西州基础理论研究项目(2018SF5018) 第一作者简介:程建平(1974-),男,湖南益阳人,中学一级,主要从事生物学研究﹒E-mail: 1040586314@qq.com﹒*通信作者简介:何则强(1974-),男,湖南益阳人,教授,博士,主要从事资源化研究﹒E-mail: csuhzq@163.com
更新日期/Last Update: 2019-10-24